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Anti-wrinkle effectiveness In Use pilot study

This study proves that after the continuous application of our pine seed oil there is a stabilization of the skin topography. This reflects a reduction of the wrinkles maximum depth and volume.

As a result the skin topography of the wrinkles becomes more tenuous, reducing the general wrinkle appearance. These results can be confirmed in the following figures.

Figure 1 – Reduction of the maximum depth of the wrinkles: 19,9%*

Figure 2 – Reduction of the volume of the wrinkles: 31,7%*

phdtrails*experimental study to demonstrate the biological effectiveness – In Use pilot anti-wrinkle effectiveness – tested on 6 (six) volunteers for 28 days with twice daily application.


Skin compatibility trials

(Human Repeated Insult Patch Test [HRIPT])

Skin compatibility trials were made to confirm the skin compatibility and the absence of sensitizing potencial of the products after repeated epicutaneous Patch aplication.

This study allows us to justify the revindication of:

  • Hypoallergenic
  • Dermatological tested


Product quality control

Chemical trials developed:

  • Solubility
  • pH
  • Relative density
  • Acidity constant


Stability trials

Chemical and microbiological trials were developed both at the initial sample and after six months in order to prove its stability and features condition.